RESTRICTION SITES TO INSIGHTS

Project Summary -

• Methodology: Utilized TASSEL Genotyping-by-Sequencing (GBS) to produce a comprehensive genotypic dataset for 471 rice germplasm taxa.
• SNP Detection: Initially identified 212,818 SNPs across the rice genome, refined to 115,250 SNPs after data normalization.
• PCA Analysis: Conducted Principal Component Analysis (PCA) to assess genetic diversity and population structure, identifying population stratification and genetic outliers.
• Dendrogram: Illustrated the branching structure of genetically similar populations and their evolutionary relationships.
• Kinship Matrix: Created a heatmap with a color gradient and dendrogram, showing three main subpopulations and varying degrees of genetic relatedness among the first 50 lines.
• Applications: The dataset supports Genome-Wide Association Studies (GWAS), Marker-Assisted Breeding (MAS), and enhances rice breeding strategies and genetic research.

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